Fiche publication
Date publication
janvier 2020
Journal
Chemical science
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah
,
Dr WAGNER Alain
,
Dr KOLODYCH Sergii
,
Dr KONIEV Sasha
Tous les auteurs :
Dovgan I, Hentz A, Koniev O, Ehkirch A, Hessmann S, Ursuegui S, Delacroix S, Riomet M, Taran F, Cianférani S, Kolodych S, Wagner A
Lien Pubmed
Résumé
Controlled protein functionalization holds great promise for a wide variety of applications. However, despite intensive research, the stoichiometry of the functionalization reaction remains difficult to control due to the inherent stochasticity of the conjugation process. Classical approaches that exploit peculiar structural features of specific protein substrates, or introduce reactive handles mutagenesis, are by essence limited in scope or require substantial protein reengineering. We herein present equimolar native chemical tagging (ENACT), which precisely controls the stoichiometry of inherently random conjugation reactions by combining iterative low-conversion chemical modification, process automation, and bioorthogonal -tagging. We discuss the broad applicability of this conjugation process to a variety of protein substrates and payloads.
Référence
Chem Sci. 2020 Jan 2;11(5):1210-1215
