Fiche publication
Date publication
juin 2021
Journal
International journal of molecular sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Pr POLETTE Myriam
,
Dr DORMOY Valerian
Tous les auteurs :
Diabasana Z, Perotin JM, Belgacemi R, Ancel J, Mulette P, Launois C, Delepine G, Dubernard X, Mérol JC, Ruaux C, Gosset P, Maskos U, Polette M, Deslée G, Dormoy V
Lien Pubmed
Résumé
The gene cluster region, CHRNA3/CHRNA5/CHRNB4, encoding for nicotinic acetylcholine receptor (nAChR) subunits, contains several genetic variants linked to nicotine addiction and brain disorders. The CHRNA5 single-nucleotide polymorphism (SNP) rs16969968 is strongly associated with nicotine dependence and lung diseases. Using immunostaining studies on tissue sections and air-liquid interface airway epithelial cell cultures, in situ hybridisation, transcriptomic and cytokines detection, we analysed rs16969968 contribution to respiratory airway epithelial remodelling and modulation of inflammation. We provide cellular and molecular analyses which support the genetic association of this polymorphism with impaired ciliogenesis and the altered production of inflammatory mediators. This suggests its role in lung disease development.
Mots clés
airway epithelial cells, differentiation, inflammation, remodelling, rs16969968
Référence
Int J Mol Sci. 2021 Jun 22;22(13):
