Comparative Study of Secreted Proteins, Enzymatic Activities of Wood Degradation and Stilbene Metabolization in Grapevine Botryosphaeria Dieback Fungi.

Fiche publication

Date publication

juillet 2021

Journal

Journal of fungi (Basel, Switzerland)

Auteurs

Membres identifiés du Cancéropôle Est :
Mme SCHAEFFER-REISS Christine

Tous les auteurs :
Labois C, Stempien E, Schneider J, Schaeffer-Reiss C, Bertsch C, Goddard ML, Chong J

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/34356948

Résumé

fungi are plant pathogens associated with Botryosphaeria dieback. To better understand the virulence factors of these fungi, we investigated the diversity of secreted proteins and extracellular enzyme activities involved in wood degradation and stilbene metabolization in and , which are two major fungi associated with grapevine B. dieback. Regarding the analysis of proteins secreted by the two fungi, our study revealed that . , known to be more aggressive than . , was characterized by a higher quantity and diversity of secreted proteins, especially hydrolases and oxidoreductases that are likely involved in cell wall and lignin degradation. In addition, when fungi were grown with wood powder, the extracellular laccase and Mn peroxidase enzyme activities were significantly higher in . compared to . Importantly, our work also showed that secreted proteins produced after grapevine wood addition are able to rapidly metabolize the grapevine stilbenes. Overall, a higher diversity of resveratrol and piceatannol metabolization products was found with enzymes of compared to This study emphasizes the diversity of secreted virulence factors found in B. dieback fungi and suggests that some resveratrol oligomers produced in grapevine wood after pathogen attack could be formed via pathogenic fungal oxidases.