Fiche publication
Date publication
janvier 2021
Journal
Frontiers in microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie
,
Dr TERRYN Christine
,
Dr VELARD Frédéric
Tous les auteurs :
Lamret F, Varin-Simon J, Velard F, Terryn C, Mongaret C, Colin M, Gangloff SC, Reffuveille F
Lien Pubmed
Résumé
species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the host immune system responses and to potential antibiotic treatments. We suggested that the periprosthetic bone environment is stressful for bacteria, influencing biofilm development. To provide insights into biofilm properties of three strains [including one methicillin-resistant (MRSA)] under this specific environment, we assessed several parameters related to bone conditions and expected to affect biofilm characteristics. We reported that the three strains harbored different behaviors in response to the lack of oxygen, casamino acids and glucose starvation, and high concentration of magnesium. Each strain presented different biofilm biomass and live adherent cells proportion, or matrix production and composition. However, the three strains shared common responses in a bone-like environment: a similar production of extracellular DNA and engagement of the SOS response. This study is a step toward a better understanding of periprosthetic joint infections and highlights targets, which could be common among strains and for future antibiofilm strategies.
Mots clés
MRSA, MSSA, biofilms, bone microenvironment, prosthetic joint infection
Référence
Front Microbiol. 2021 ;12:714994
