Fiche publication
Date publication
septembre 2021
Journal
Microorganisms
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie
Tous les auteurs :
Varin-Simon J, Lamret F, Colin M, Gangloff SC, Mongaret C, Reffuveille F
Lien Pubmed
Résumé
The study of biofilms in vitro is complex and often limited by technical problems due to simplified models. Here, we compared biofilm formation, from species involved in bone and prosthesis infection, in a static model with a dynamic model. Using similar parameters, the percentage of live bacteria within the biofilm was higher in dynamic than in static approach. In both models, bacterial internalization in osteoblast-like cells, playing the role of stress factor, affected this proportion but in opposite ways: increase of live bacteria proportion in the static model (×2.04 ± 0.53) and of dead bacteria proportion (×3.5 ± 1.03) in the dynamic model. This work highlights the huge importance in the selection of a relevant biofilm model in accordance with the environmental or clinical context to effectively improve the understanding of biofilms and the development of better antibiofilm strategies.
Mots clés
Cutibacterium acnes, biofilm models, host-pathogen interactions
Référence
Microorganisms. 2021 Sep 26;9(10):
