Fiche publication
Date publication
novembre 2021
Journal
Nanoscale
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MENARD-MOYON Cécilia
,
Dr BIANCO Alberto
Tous les auteurs :
Orecchioni M, Fusco L, Mall R, Bordoni V, Fuoco C, Rinchai D, Guo S, Sainz R, Zoccheddu M, Gurcan C, Yilmazer A, Zavan B, Ménard-Moyon C, Bianco A, Hendrickx W, Bedognetti D, Delogu LG
Lien Pubmed
Résumé
We recently found by single-cell mass cytometry that human B cells internalize graphene oxide (GO). The functional impact of such uptake on B cells remains unexplored. Here, we disclosed the effects of GO and amino-functionalized GO (GONH) interacting with human B cells and at the protein and gene expression levels. Moreover, our study considered three different subpopulations of B cells and their functionality in terms of: (i) cytokine production, (ii) activation markers, (iii) killing activity towards cancer cells. Single-cell mass cytometry screening revealed the higher impact of GO on cell viability towards naïve, memory, and plasma B cell subsets. Different cytokines such as granzyme B (GrB) and activation markers, like CD69, CD80, CD138, and CD38, were differently regulated by GONH compared to GO, supporting possible diverse B cell activation paths. Moreover, co-culture experiments also suggest the functional ability of both GOs to activate B cells and therefore enhance the toxicity towards HeLa cancer cell line. Complete transcriptomic analysis on a B cell line highlighted the distinctive GO and GONH elicited responses, inducing pathways such as B cell receptor and CD40 signaling pathways, key players for GrB secretion. B cells were regularly left behind the scenes in graphene biological studies; our results may open new horizons in the development of GO-based immune-modulatory strategies having B cell as main actors.
Référence
Nanoscale. 2021 Nov 19;:
