Anti-inflammatory effects of free and liposome-encapsulated natural Algerian thermal water in RAW 264.7 macrophages.

Fiche publication

Date publication

janvier 2022

Journal

International journal of pharmaceutics

Auteurs

Membres identifiés du Cancéropôle Est :
Pr FOURNEL Sylvie , Dr FRISCH Benoit , Dr HEURTAULT Béatrice

Tous les auteurs :
Mokdad R, Seguin C, Fournel S, Frisch B, Heurtault B, Hadjsadok A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/35007687

Résumé

The main objectives of this work were to formulate liposomes encapsulating highly mineralized thermal waters (TWs) and to study anti-inflammatory effect of free and encapsulated thermal waters on RAW 264.7 macrophage cells stimulated with lipopolysaccharide (LPS). TWs-loaded conventional and deformable liposomes (TWs-Lip and TWs-DLip) were prepared by sonication and extrusion, respectively. They were considered for their vesicle size, zeta potential, entrapment efficiency, physical stability and in vitro anti-inflammatory effect. Formulated liposome suspensions have a low polydispersity and nanometric size range with zeta potential values close to zero. The vesicle size was stable for 30 days. Entrapment efficiency of TWs was above 90 % in conventional liposomes and 70 % in deformable liposomes. Pretreatment of LPS-stimulated murine macrophages, with free and liposome-encapsulated TWs, resulted in a significant reduction in nitric oxide (NO) production and modulated tumor necrosis factor-α (TNF-α) production suggesting an anti-inflammatory effect which was even more striking with TWs-Lip and TWs-DLip. Liposome formulations may offer a suitable approach for transdermal delivery of TWs, indicated in inflammatory skin diseases.