Fiche publication
Date publication
janvier 2022
Journal
Biomedicines
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOULAGNON-ROMBI Camille
,
Dr AUDONNET Sandra
,
Dr KERDJOUDJ Halima
,
Dr GINDRAUX Florelle
Tous les auteurs :
Dubus M, Scomazzon L, Chevrier J, Ledouble C, Baldit A, Braux J, Gindraux F, Boulagnon C, Audonnet S, Colin M, Rammal H, Mauprivez C, Kerdjoudj H
Lien Pubmed
Résumé
Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton's jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive ( and ) and Gram negative ( and ) growth and adhesion. Although DC-WJ activated the neutrophils and monocytes in a comparable magnitude to DV-WJ, macrophages modulated their phenotypes and polarization states from the resting M0 phenotype to the hybrid M1/M2 phenotype in the presence of DC-WJ. M1 phenotype was predominant in the presence of DV-WJ. Finally, the subcutaneous implantation of DC-WJ showed total resorption after three weeks of implantation without any sign of foreign body reaction. These significant data shed light on the potential regenerative application of DC-WJ in providing a suitable biomaterial for tissue regenerative medicine and an ideal strategy to prevent wound-associated infections.
Mots clés
Wharton’s jelly, antibacterial, bioactivity, decellularization, immunomodulation
Référence
Biomedicines. 2022 Jan 21;10(2):
