In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata.

Date publication

janvier 2022

Journal

Methods in molecular biology (Clifton, N.J.)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr MUTTERER Jérôme , Dr HEINLEIN Manfred

Tous les auteurs :
Huang C, Mutterer J, Heinlein M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/35349138

Résumé

The deposition and turnover of callose (beta-1,3 glucan polymer) in the cell wall surrounding the neck regions of plasmodesmata (PD) controls the cell-to-cell diffusion rate of molecules and, therefore, plays an important role in the regulation of intercellular communication in plants.Here we describe a simple and fast in vivo staining procedure for the imaging and quantification of callose at PD. We also introduce calloseQuant, a plug-in for semiautomated image analysis and non-biased quantification of callose levels at PD using ImageJ.

Mots clés

Aniline blue, Callose, Confocal microscopy, ImageJ, Plasmodesmata, flg22

Référence

Methods Mol Biol. 2022 ;2457:151-165