Imaging and Measuring Vesicular Acidification with a Plasma Membrane-Targeted Ratiometric pH Probe.

Date publication

avril 2022

Journal

Analytical chemistry

Auteurs

Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey

Tous les auteurs :
Michelis S, Danglot L, Vauchelles R, Klymchenko AS, Collot M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/35377610

Résumé

Tracking the pH variation of intracellular vesicles throughout the endocytosis pathway is of prior importance to better assess the cell trafficking and metabolism of cells. Small molecular fluorescent pH probes are valuable tools in bioimaging but are generally not targeted to intracellular vesicles or are directly targeted to acidic lysosomes, thus not allowing the dynamic observation of the vesicular acidification. Herein, we designed Mem-pH, a fluorogenic ratiometric pH probe based on chromenoquinoline with appealing photophysical properties, which targets the plasma membrane (PM) of cells and further accumulates in the intracellular vesicles by endocytosis. The exposition of Mem-pH toward the vesicle's lumen allowed to monitor the acidification of the vesicles throughout the endocytic pathway and enabled the measurement of their pH via ratiometric imaging.

Référence

Anal Chem. 2022 Apr 4;: