Aerosol-Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction.

Date publication

avril 2022

Journal

Nanomaterials (Basel, Switzerland)

Auteurs

Membres identifiés du Cancéropôle Est :
Pr RIHN Bertrand

Tous les auteurs :
Leroux MM, Hocquel R, Bourge K, Kokot B, Kokot H, Koklič T, Štrancar J, Ding Y, Kumar P, Schmid O, Rihn BH, Ferrari L, Joubert O

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/35458071

Résumé

Nanoparticle toxicity assessments have moved closer to physiological conditions while trying to avoid the use of animal models. An example of new in vitro exposure techniques developed is the exposure of cultured cells at the air-liquid interface (ALI), particularly in the case of respiratory airways. While the commercially available VITROCELL Cloud System has been applied for the delivery of aerosolized substances to adherent cells under ALI conditions, it has not yet been tested on lung surfactant and semi-adherent cells such as alveolar macrophages, which are playing a pivotal role in the nanoparticle-induced immune response.

Mots clés

NR8383, RNA extraction, VITROCELL® Cloud System, air–liquid interface, macrophages, nanoparticles, surfactant, transcriptomic

Référence

Nanomaterials (Basel). 2022 Apr 15;12(8):