Polynucleotide differentiation using hybrid solid-state nanopore functionalizing with α-hemolysin.

Date publication

janvier 2020

Journal

Soft matter

Auteurs

Membres identifiés du Cancéropôle Est :
Dr PICAUD Fabien

Tous les auteurs :
Bentin J, Balme S, Picaud F

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/31853534

Résumé

We report results from full atomistic molecular dynamics simulations on the properties of biomimetic nanopores. This latter result was obtained through the direct insertion of an α-hemolysin protein inside a hydrophobic solid-state nanopore. Upon translocation of different DNA strands, we demonstrate here that the theoretical system presents the same discrimination properties as the experimental one obtained previously. This opens an interesting way to promote the stability of a specific protein inside a solid nanopore to develop further biomimetic applications for DNA or protein sequencing.

Mots clés

Amino Acid Sequence, genetics, Biomimetics, DNA, chemistry, Hemolysin Proteins, chemistry, Hydrophobic and Hydrophilic Interactions, Molecular Dynamics Simulation, Nanopores, ultrastructure, Polynucleotides, chemistry

Référence

Soft Matter. 2020 Jan 28;16(4):1002-1010