Fiche publication
Date publication
janvier 2022
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JACOB Christophe
,
Pr WEISSMAN Kira
Tous les auteurs :
Massicard JM, Su L, Jacob C, Weissman KJ
Lien Pubmed
Résumé
The CRISPR/Cas system, which has been widely applied to organisms ranging from microbes to animals, is currently being adapted for use in Streptomyces bacteria. In this case, it is notably applied to rationally modify the biosynthetic pathways giving rise to the polyketide natural products, which are heavily exploited in the medical and agricultural arenas. Our aim here is to provide the potential user with a practical guide to exploit this approach for manipulating polyketide biosynthesis, by treating key experimental aspects including vector choice, design of the basic engineering components, and trouble-shooting.
Mots clés
CRISPR/Cas, Genome editing, Polyketide biosynthesis, Streptomyces
Référence
Methods Mol Biol. 2022 ;2489:173-200
