Fiche publication
Date publication
décembre 2016
Journal
Journal of clinical microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr VILLENA Isabelle
Tous les auteurs :
Villard O, Cimon B, L'Ollivier C, Fricker-Hidalgo H, Godineau N, Houze S, Paris L, Pelloux H, Villena I, Candolfi E
Lien Pubmed
Résumé
Toxoplasmosis, a benign infection, is asymptomatic or paucisymptomatic in over 80% of cases, except in immunocompetent patients suffering from ocular toxoplasmosis or in immunocompromised patients with opportunistic or congenital toxoplasmosis. Diagnosis is based mainly on serology testing. Thus, we compared the performance of the nine most commonly used commercial automated or semiautomated immunoassays for IgG and IgM Toxoplasma gondii antibody detection, that is, the Advia Centaur, Architect, AxSYM, Elecsys, Enzygnost, Liaison, Platelia, VIDAS, and VIDIA assays. The assays were conducted on four panels of serum samples derived during routine testing from patients with an interfering disease and who exhibited a low IgG antibody level in one of two clinical settings, namely, acute or chronic toxoplasmosis. As a result, IgG sensitivities ranged from 97.1% to 100%, and IgG specificities ranged from 99.5% to 100%. For IgG quantification, strong differences in IgG titers (expressed in IU/ml) were noted depending on the assay used. IgM sensitivities ranged from 65% to 97.9%, and IgM specificities ranged from 92.6% to 100%. For defining the best serological strategies to be implemented, it appears crucial to compare the diagnostic performance of the different tests with respect to their specificity and sensitivity in detecting the presence of IgG and IgM antibodies.
Mots clés
Antibodies, Protozoan, blood, Automation, Laboratory, methods, Humans, Immunoassay, methods, Immunocompromised Host, Immunoglobulin G, blood, Immunoglobulin M, blood, Sensitivity and Specificity, Toxoplasma, immunology, Toxoplasmosis, diagnosis
Référence
J Clin Microbiol. 2016 12;54(12):3034-3042
