What is the best technic to dislodge Staphylococcus epidermidis biofilm on medical implants?

Date publication

août 2022

Journal

BMC microbiology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr ZWETYENGA Narcisse

Tous les auteurs :
Moris V, Lam M, Amoureux L, Magallon A, Guilloteau A, Maldiney T, Zwetyenga N, Falentin-Daudre C, Neuwirth C

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/35933363

Résumé

Bacterial biofilm can occur on all medical implanted devices and lead to infection and/or dysfunction of the device. In this study, artificial biofilm was formed on four different medical implants (silicone, piccline, peripheral venous catheter and endotracheal tube) of interest for our daily clinical and/or research practice. We investigated the best conventional technic to dislodge the biofilm on the implants and quantified the number of bacteria. Staphylococcus epidermidis previously isolated from a breast implant capsular contracture on a patient in the university hospital of Dijon was selected for its ability to produce biofilm on the implants. Different technics (sonication, Digest-EUR®, mechanized bead mill, combination of sonication plus Digest-EUR®) were tested and compared to detach the biofilm before quantifying viable bacteria by colony counting.

Mots clés

Biofilm quantification, Endotracheal tube, Enzymatic treatment, Medical implant, Peripheral venous catheter, Piccline, Silicone, Sonication, Staphylococcus epidermidis

Référence

BMC Microbiol. 2022 08 6;22(1):192