Impact of the structure of biocompatible aliphatic polycarbonates on siRNA transfection ability.

Date publication

mars 2015

Journal

Biomacromolecules

Auteurs

Membres identifiés du Cancéropôle Est :
Dr PEIXOTO Paul

Tous les auteurs :
Frère A, Kawalec M, Tempelaar S, Peixoto P, Hendrick E, Peulen O, Evrard B, Dubois P, Mespouille L, Mottet D, Piel G

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/25603322

Résumé

RNAi therapeutics are promising therapeutic tools that have sparked the interest of many researchers. In an effort to provide a safe alternative to PEI, we have designed a series of new guanidinium- and morpholino-functionalized biocompatible and biodegradable polycarbonate vectors. The impact of different functions (morpholino-, guanidinium-, hydrophobic groups) of the architecture (linear homopolymer to dumbbell-shape) and of the molecular weight of these copolymers on their capacity to form polyplexes and to decrease the expression of two epigenetic regulators of gene expression, HDAC7 and HDAC5, was evaluated. The use of one of these polymers combining morpholine and guanidine functions at the ratio >1 and hydrophobic trimethylene carbonate groups showed a significant decrease of mRNA and protein level in HeLa cells, similar to PEI. These results highlight the potential of polycarbonate vectors for future in vivo application as an anticancer therapy.

Mots clés

Carbonates, chemistry, Gene Expression, Gene Knockdown Techniques, HeLa Cells, Histone Deacetylases, genetics, Humans, Polymers, chemistry, RNA Interference, RNA, Small Interfering, genetics, Transfection

Référence

Biomacromolecules. 2015 Mar;16(3):769-79