Fiche publication
Date publication
septembre 2022
Journal
Cells
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BELJEBBAR Abdelilah
,
Dr KERDJOUDJ Halima
,
Pr ALEM-MARCHAND Halima
Tous les auteurs :
Dubus M, Scomazzon L, Ledouble C, Braux J, Beljebbar A, Van Gulick L, Baldit A, Gorin C, Alem H, Bouland N, Britton M, Schiavi J, Vaughan TJ, Mauprivez C, Kerdjoudj H
Lien Pubmed
Résumé
In craniofacial bone defects, the promotion of bone volume augmentation remains a challenge. Finding strategies for bone regeneration such as combining resorbable minerals with organic polymers would contribute to solving the bone volume roadblock. Here, dicalcium phosphate dihydrate, chitosan and hyaluronic acid were used to functionalize a bone-side collagen membrane. Despite an increase in the release of inflammatory mediators by human circulating monocytes, the in vivo implantation of the functionalized membrane allowed the repair of a critical-sized defect in a calvaria rat model with de novo bone exhibiting physiological matrix composition and structural organization. Microtomography, histological and Raman analysis combined with nanoindentation testing revealed an increase in bone volume in the presence of the functionalized membrane and the formation of woven bone after eight weeks of implantation; these data showed the potential of dicalcium phosphate dihydrate, chitosan and hyaluronic acid to induce an efficient repair of critical-sized bone defects and establish the importance of thorough multi-scale characterization in assessing biomaterial outcomes in animal models.
Mots clés
bone volume augmentation, critical-sized bone defect, dicalcium phosphate dihydrate, hybrid bioactive materials, multi-scale characterization, polysaccharides
Référence
Cells. 2022 09 14;11(18):
