Fiche publication
Date publication
janvier 2015
Journal
Virus research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MUTTERER Jérôme
,
Dr ERHARDT Mathieu
Tous les auteurs :
Pakdel A, Mounier C, Klein E, Hleibieh K, Monsion B, Mutterer J, Erhardt M, Bouzoubaa S, Ratti C, Gilmer D
Lien Pubmed
Résumé
Beet necrotic yellow vein virus (BNYVV) is a multipartite positive-strand RNA virus. BNYVV RNA-1 encodes a non-structural p237 polyprotein processed in two proteins (p150 and p66) by a cis-acting protease activity. BNYVV non-structural proteins are closely related to replication proteins of positive strand RNA viruses such as hepeviruses rather to other plant virus replicases. The p237 and dsRNA have been localized by TEM in ER structures of infected leaf cells whereas dsRNA was immunolabeled in infected protoplasts. The p150 contains domains with methyltransferase, protease, helicase and two domains of unknown function whereas p66 encompasses the RNA-dependent RNA-polymerase signature. We report the existing interactions between functional domains of the p150 and p66 proteins and the addressing of the benyvirus replicase to the endoplasmic reticulum. Yeast two-hybrid approach, colocalization with FRET-FLIM analyses and co-immunoprecipitation highlighted existing interactions that suggest the presence of a multimeric complex at the vicinity of the cellular membranous web.
Mots clés
Endoplasmic Reticulum, metabolism, Gene Expression, Intracellular Space, metabolism, Plant Diseases, virology, Plant Viruses, physiology, Protein Binding, Protein Interaction Domains and Motifs, genetics, Protein Transport, Protoplasts, metabolism, RNA Replicase, chemistry, RNA, Double-Stranded, genetics, RNA, Viral, genetics
Référence
Virus Res.. 2015 Jan 22;196:94-104
