Fiche publication
Date publication
janvier 2015
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine
Tous les auteurs :
Sima F, Davidson PM, Dentzer J, Gadiou R, Pauthe E, Gallet O, Mihailescu IN, Anselme K
Lien Pubmed
Résumé
The lifetime of bone implants inside the human body is directly related to their osseointegration. Ideally, future materials should be inspired by human tissues and provide the material structure-function relationship from which synthetic advanced biomimetic materials capable of replacing, repairing, or regenerating human tissues can be produced. This work describes the development of biomimetic thin coatings on titanium implants to improve implant osseointegration. The assembly of an inorganic-organic biomimetic structure by UV laser pulses is reported. The structure consists of a hydroxyapatite (HA) film grown onto a titanium substrate by pulsed-laser deposition (PLD) and activated by a top fibronectin (FN) coating deposited by matrix-assisted pulsed laser evaporation (MAPLE). A pulsed KrF* laser source (λ = 248 nm, τ = 25 ns) was employed at fluences of 7 and 0.7J/cm(2) for HA and FN transfer, respectively. Films approximately 1500 and 450 nm thick were obtained for HA and FN, respectively. A new cryogenic temperature-programmed desorption mass spectrometry analysis method was employed to accurately measure the quantity of immobilized protein. We determined that less than 7 μg FN per cm(2) HA surface is adequate to improve adhesion, spreading, and differentiation of osteoprogenitor cells. We believe that the proposed fabrication method opens the door to combining and immobilizing two or more inorganic and organic materials on a solid substrate in a well-defined manner. The flexibility of this method enables the synthesis of new hybrid materials by simply tailoring the irradiation conditions according to the thermo-physical properties of the starting materials.
Mots clés
Biomimetics, Bone and Bones, cytology, Cell Adhesion, Cell Line, Cell Proliferation, Coated Materials, Biocompatible, chemistry, Durapatite, chemistry, Fibronectins, chemistry, Humans, Lasers, Mass Spectrometry, Materials Testing, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Organic Chemicals, chemistry, Osseointegration, Osteoblasts, cytology, Prostheses and Implants, Prosthesis Design, Protein Binding, Regeneration, Spectrophotometry, Stem Cells, cytology, Surface Properties, Titanium, chemistry, Ultraviolet Rays
Référence
ACS Appl Mater Interfaces. 2015 Jan;7(1):911-20
