Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status.

Date publication

mars 2011

Journal

BMC cell biology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr DE CARVALHO BITTENCOURT Marcelo , Dr DE ISLA Natalia

Tous les auteurs :
Basciano L, Nemos C, Foliguet B, de Isla N, de Carvalho M, Tran N, Dalloul A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/21450070

Résumé

In the bone marrow, hematopietic and mesenchymal stem cells form a unique niche in which the oxygen tension is low. Hypoxia may have a role in maintaining stem cell fate, self renewal and multipotency. However, whereas most studies addressed the effect of transient in vitro exposure of MSC to hypoxia, permanent culture under hypoxia should reflect the better physiological conditions.

Mots clés

Cell Count, Cell Differentiation, physiology, Cell Hypoxia, Cell Proliferation, Cells, Cultured, Flow Cytometry, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Humans, Mesenchymal Stem Cells, cytology, Microscopy, Electron, Multipotent Stem Cells, cytology, Stem Cell Research

Référence

BMC Cell Biol.. 2011 Mar 30;12:12