A lipid transfer protein ensures nematode cuticular impermeability.

Fiche publication

Date publication

novembre 2022

Journal

iScience

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BIRCK Catherine , Dr POTERSZMAN Arnaud

Tous les auteurs :
Njume FN, Razzauti A, Soler M, Perschin V, Fazeli G, Bourez A, Delporte C, Ghogomu SM, Poelvoorde P, Pichard S, Birck C, Poterszman A, Souopgui J, Van Antwerpen P, Stigloher C, Vanhamme L, Laurent P

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/36339267

Résumé

The cuticle of is impermeable to chemicals, toxins, and pathogens. However, increased permeability is a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute to the permeability barrier. Here, we identify the lipid transfer protein GMAP-1 as a critical element setting the permeability of the cuticle. A deletion mutant increases cuticular permeability to sodium azide, levamisole, Hoechst, and DiI. Expressing GMAP-1 in the hypodermis or transiently in the adults is sufficient to rescue this permeability phenotype. GMAP-1 protein is secreted from the hypodermis to the aqueous fluid filling the space between collagen fibers of the cuticle. , GMAP-1 protein binds phosphatidylserine and phosphatidylcholine while , GMAP-1 sets the surface lipid composition and organization Altogether, our results suggest GMAP-1 secreted by hypodermis shuttles lipids to the surface to form the permeability barrier of .