Effects of industrially produced 2-dimensional molybdenum disulfide materials in primary human basophils.

Fiche publication

Date publication

janvier 2023

Journal

NanoImpact

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BIANCO Alberto

Tous les auteurs :
Lin H, Del Rio Castillo AE, González VJ, Jacquemin L, Panda JK, Bonaccorso F, Vázquez E, Bianco A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/36626980

Résumé

MoS has been increasingly used in place of graphene as a flexible and multifunctional 2D material in many biomedical applications such as cancer detection and drug delivery, which makes it crucial to evaluate downstream compatibility in human immune cells. Molybdenum is a component of stainless-steel stent implants and has previously been implicated in stent hypersensitivity. In view of this, it is important to ascertain the effect of MoS on allergy-relevant cells. Basophils are a less commonly used immune cell type. Unlike mast cells, basophils can be easily derived from primary human blood and can act as a sentinel for allergy. However, merely testing any one type of MoS in basophils could result in different biological results. We thus decided to compare 2D MoS from the two companies BeDimensional© (BD) and Biograph Solutions (BS), manufactured with two different but commonly exploited methods (BD, deoxycholate surfactant in a high-pressure liquid exfoliation, and BS using glycine in ball-milling exfoliation) to elucidate immunological end-points common to both MoS and to demonstrate the need for biological verification for end-users who may require a change of supplier. We report higher histamine production in human basophils with MoS. No effects on either surface basophil activation markers CD63 and CD203c or reactive oxygen species (ROS) production and cell viability were observed. However, different cytokine production patterns were evidenced. IL-6 and IL-1β but not TNF and GM-CSF were increased for both MoS. BS-MoS increased IL-4, while BD-MoS decreased IL-4 and increased IL-13. Molybdate ion itself only increased IL-1β and IL-4. Deoxycholate surfactant decreased viability at 18 h and increased ROS upon basophil activation. Therefore, these results demonstrate the safety of MoS in human basophils in general and highlight the importance of considering manufacturer additives and variability when selecting and investigating 2D materials such as MoS.