Fiche publication
Date publication
février 2023
Journal
Frontiers in microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ENNIFAR Eric
Tous les auteurs :
Giuliodori AM, Belardinelli R, Duval M, Garofalo R, Schenckbecher E, Hauryliuk V, Ennifar E, Marzi S
Lien Pubmed
Résumé
CspA is an RNA binding protein that accumulates during cold-shock and stimulates translation of several mRNAs-including its own. Translation in the cold of mRNA involves a cis-acting thermosensor element, which enhances ribosome binding, and the trans-acting action of CspA. Using reconstituted translation systems and probing experiments we show that, at low temperature, CspA specifically promotes the translation of the mRNA folded in the conformation less accessible to the ribosome, which is formed at 37°C but is retained upon cold shock. CspA interacts with its mRNA without inducing large structural rearrangements, but allowing the progression of the ribosomes during the transition from translation initiation to translation elongation. A similar structure-dependent mechanism may be responsible for the CspA-dependent translation stimulation observed with other probed mRNAs, for which the transition to the elongation phase is progressively facilitated during cold acclimation with the accumulation of CspA.
Mots clés
CspA, RNA chaperone, cold-shock, ribosome, translation regulation
Référence
Front Microbiol. 2023 02 9;14:1118329
