Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS.

Fiche publication

Date publication

janvier 2023

Journal

STAR protocols

Auteurs

Membres identifiés du Cancéropôle Est :
Pr GHIRINGHELLI François , Dr LADOIRE Sylvain , Dr LIMAGNE Emeric , Dr GALLAND Loïck

Tous les auteurs :
Kalfeist L, Petit S, Galland L, Ghiringhelli F, Ladoire S, Limagne E

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/36856770

Résumé

The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45 tumor-infiltrating lymphocytes, cancer-associated fibroblasts, and tumor cells. The protocol includes four steps: generation of 4T1 tumors, tumor collection and digestion, magnetic sorting of the different populations, and phenotypic validation of sorted cells. For complete details on the use and execution of this protocol, please refer to Limagne et al. (2022)..