The Effect of Acidic and Alkaline Seawater on the F-Actin-Dependent Ca Signals Following Insemination of Immature Starfish Oocytes and Mature Eggs.

Fiche publication

Date publication

février 2023

Journal

Cells

Auteurs

Membres identifiés du Cancéropôle Est :
Pr LEHN Jean-Marie

Tous les auteurs :
Limatola N, Chun JT, Schneider SC, Schmitt JL, Lehn JM, Santella L

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/36899875

Résumé

In starfish, the addition of the hormone 1-methyladenine (1-MA) to immature oocytes (germinal vesicle, GV-stage) arrested at the prophase of the first meiotic division induces meiosis resumption (maturation), which makes the mature eggs able to respond to the sperm with a normal fertilization response. The optimal fertilizability achieved during the maturation process results from the exquisite structural reorganization of the actin cytoskeleton in the cortex and cytoplasm induced by the maturing hormone. In this report, we have investigated the influence of acidic and alkaline seawater on the structure of the cortical F-actin network of immature oocytes of the starfish () and its dynamic changes upon insemination. The results have shown that the altered seawater pH strongly affected the sperm-induced Ca response and the polyspermy rate. When immature starfish oocytes were stimulated with 1-MA in acidic or alkaline seawater, the maturation process displayed a strong dependency on pH in terms of the dynamic structural changes of the cortical F-actin. The resulting alteration of the actin cytoskeleton, in turn, affected the pattern of Ca signals at fertilization and sperm penetration.