Absorption/Attenuation Spectral Description of ESKAPEE Bacteria: Application to Seeder-Free Culture Monitoring, Mammalian T-Cell and Bacteria Mixture Analysis and Contamination Description.

Fiche publication

Date publication

avril 2023

Journal

Sensors (Basel, Switzerland)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr WACOGNE Bruno

Tous les auteurs :
Wacogne B, Belinger Podevin M, Vaccari N, Koubevi C, Codjiová C, Gutierrez E, Bourgeois P, Davoine L, Robert-Nicoud M, Rouleau A, Frelet-Barrand A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/37177529

Résumé

Despite numerous innovations, measuring bacteria concentrations on a routine basis is still time consuming and ensuring accurate measurements requires careful handling. Furthermore, it often requires sampling small volumes of bacteria suspensions which might be poorly representative of the real bacteria concentration. In this paper, we propose a spectroscopy measurement method based on a description of the absorption/attenuation spectra of ESKAPEE bacteria. Concentrations were measured with accuracies less than 2%. In addition, mixing the mathematical description of the absorption/attenuation spectra of mammalian T-cells and bacteria allows for the simultaneous measurements of both species' concentrations. This method allows real-time, sampling-free and seeder-free measurement and can be easily integrated into a closed-system environment.