Fiche publication
Date publication
juillet 2023
Journal
Lab on a chip
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BORG Christophe
,
Dr BOLOPION Aude
,
Dr LOYON Romain
,
Dr GAUTHIER Michaël
Tous les auteurs :
Lipp C, Koebel L, Loyon R, Bolopion A, Spehner L, Gauthier M, Borg C, Bertsch A, Renaud P
Lien Pubmed
Résumé
The understanding of cell-cell and cell-matrix interactions receptor and ligand binding relies on our ability to study the very first events of their contact. Of particular interest is the interaction between a T cell receptor and its cognate peptide-major histocompatibility complex. Indeed, analyzing their binding kinetics and cellular avidity in large-scale low-cost and fast cell sorting would largely facilitate the access to cell-based cancer immunotherapies. We thus propose a microfluidic tool able to independently control two types of micro-sized objects, put them in contact for a defined time and probe their adhesion state. The device consists of hydrodynamic traps holding the first type of cell from below against the fluid flow, and a dielectrophoretic system to force the second type of object to remain in contact with the first one. First, the device is validated by performing an adhesion frequency assay between fibroblasts and fibronectin coated beads. Then, a study is conducted on the modification of the cellular environment to match the dielectrophoretic technology requirements without modifying the cell viability and interaction functionalities. Finally, we demonstrate the capability of the developed device to put cancer cells and a population of T cells in contact and show the discrimination between specific and non-specific interactions based on the pair lifetime. This proof-of-concept device lays the foundations for the development of next generation fast cell-cell interaction technologies.
Référence
Lab Chip. 2023 07 17;:
