Novel role of fluorescent in situ hybridization technique (FISH) in recommended surgical margins of dermatofibrosarcoma protuberans: A preliminary study.

Fiche publication

Date publication

août 2023

Journal

Annales de chirurgie plastique et esthetique

Auteurs

Membres identifiés du Cancéropôle Est :
Pr CALLIER Patrick

Tous les auteurs :
Hallier A, Callier P, Sauge J, Cristofari S, Lombardo GAG, Aubriot-Lorton MH, Stivala A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/37652836

Résumé

Dermatofibrosarcoma protuberans (DFSP) is a rare skin tumor. There is no standard recommendation for its surgical management. The currently used histological analysis are HES (hematoxylin eosin saffron) staining and immunohistochemistry for CD34 expression in particular cases. Fluorescent in situ hybridization (FISH) technique is only used to qualify the DFSP as translocated or non-translocated and is not used as a diagnostic method. The aim of our study was to determine by FISH (as a diagnostic method) whether cancerous cells that could not be identified through HES staining±immunohistochemistry were present at the two-centimeter margins that were found to be tumor-free.