Fiche publication
Date publication
septembre 2023
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain
,
Dr RYCKELYNCK Mickael
,
Dr CHAUBET Guilhem
Tous les auteurs :
Dufossez R, Krafft MP, Ursuegui S, Mosser M, Mouftakhir S, Pernod K, Chaubet G, Ryckelynck M, Wagner A
Lien Pubmed
Résumé
Droplet-based microfluidics is leading the development of miniaturized, rapid, and sensitive version of enzyme-linked immunosorbent assays (ELISAs), a central method for protein detection. These assays involve the use of a functionalized surface able to selectively capture the desired analyte. Using the droplet's oil water interface as a capture surface requires designing custom-perfluorinated fluorosurfactants bearing azide-containing polar groups, which spontaneously react when forming the droplet with strain-alkyne-functionalized antibodies solubilized in the aqueous phase. In this article, we present our research on the influence of the structure of surfactant's hydrophilic heads on the efficiency of SPAAC functionalization and on the effect of this antibody grafting process on droplet stability. We have shown that while short linkers lead to high grafting efficiency, long linkers lead to high stability, and that an intermediate size is required to balance both parameters. In the described family of surfactants, the optimal structure proved to be a PEG linker connecting a polar di-azide head and a per-fluoropolyether tail (Krytox). We also found that grafting an increasing amount of antibody, thus increasing interface coverage, increases droplet stability. It thus appears that such a bi-partite system with a reactive fluoro-surfactant in the oil phase and reactive antibody counterpart in the aqueous phase gives access in situ to novel surfactant construct providing unexplored interface structures and droplet functionality.
Mots clés
antibody conjugation, click chemistry, droplet microfluidics, emulsion stability, surface functionalization, surfactant
Référence
ACS Appl Mater Interfaces. 2023 09 13;:
