Fiche publication
Date publication
décembre 2023
Journal
RNA (New York, N.Y.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLAHOLZ Bruno
,
Dr ROMBY Pascale
Tous les auteurs :
Bahena-Ceron R, Teixeira C, Jaramillo Ponce JR, Wolff P, Couzon F, François P, Klaholz B, Vandenesch F, Romby P, Moreau K, Marzi S
Lien Pubmed
Résumé
rRNA modifications play crucial roles in fine-tuning the delicate balance between translation speed and accuracy, yet the underlying mechanisms remain elusive. Comparative analyses of the ribosomal RNA modifications in taxonomically distant bacteria could help define their general, as well as species-specific, roles. In this study, we identified a new methyltransferase, RlmQ, in responsible for the Gram-positive specific mG2601, which is not modified in (G2574). We also demonstrate the absence of methylation on C1989, equivalent to C1962, which is methylated at position 5 by the Gram-negative specific RlmI methyltransferase, a paralogue of RlmQ. Both modifications ( mG2601 and mC1962) are situated within the same tRNA accommodation corridor, hinting at a potential shared function in translation. Inactivation of Q causes the loss of methylation at G2601 and significantly impacts growth, cytotoxicity, and biofilm formation. These findings unravel the intricate connections between rRNA modifications, translation, and virulence in pathogenic Gram-positive bacteria.
Mots clés
RNA modification, Staphylococcus aureus virulence, m7G in 23S rRNA, methyltransferase, tRNA accommodation
Référence
RNA. 2023 12 19;:
