Biochemical and morpho-mechanical properties, and structural organization of rat tail tendon collagen in diet-induced obesity model.

Fiche publication

Date publication

novembre 2023

Journal

International journal of biological macromolecules

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BELJEBBAR Abdelilah , Pr GILLERY Philippe , Pr MORJANI Hamid

Tous les auteurs :
Van Gulick L, Saby C, Mayer C, Fossier E, Jaisson S, Okwieka A, Gillery P, Chenais B, Mimouni V, Morjani H, Beljebbar A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/37939767

Résumé

We have investigated the impact of obesity on the structural organization, morpho-mechanical properties of collagen fibers from rat tail tendon fascicles (RTTFs). Polarized Raman microspectroscopy showed that the collagen bands 855, 875, 938, and 960 cm as well as those 1631 and 1660 cm were affected by diet. Mechanical properties exhibited an increase in the yield strength from control (CTRL) to high fat (HF) diet (9.60 ± 1.71 and 13.09 ± 1.81 MPa) (p < 0.01) and ultimate tensile strength (13.12 ± 2.37 and 18.32 ± 2.83 MPa) (p < 0.05) with no significant change in the Young's Modulus. During mechanical, the band at 875 cm exhibited the most relevant frequency shift (2 cm). The intensity of those at 855, 875, and 938 cm in HF collagen displayed a comparable response to mechanical stress as compared to CTRL collagen with no significant diet-related changes in the Full Width at Half Maximum. Second harmonic generation technique revealed i) similar fiber straightness (0.963 ± 0.004 and 0.965 ± 0.003) and ii) significant changes in fibers diameter (1.48 ± 0.07 and 1.52 ± 0.08 μm) (p < 0.05) and length (22.06 ± 2.38 and 29.00 ± 3.76 μm) (p < 0.001) between CTRL and HF diet, respectively. The quantification of advanced glycation end products (AGEs) revealed an increase in both carboxymethyl-lysine and total fluorescence AGEs from CTRL to HF RTTFs.