Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry.

Fiche publication

Date publication

octobre 2023

Journal

STAR protocols

Auteurs

Membres identifiés du Cancéropôle Est :
Pr GHIRINGHELLI François , Dr LIMAGNE Emeric , Dr THIBAUDIN Marion

Tous les auteurs :
Daumoine S, Hampe L, Limagne E, Ghiringhelli F, Thibaudin M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/37910510

Résumé

Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al..