Fiche publication


Date publication

mai 2024

Journal

Nature communications

Auteurs

Membres identifiés du Cancéropôle Est :
Dr MUTTERER Jérôme


Tous les auteurs :
Shi B, Felipo-Benavent A, Cerutti G, Galvan-Ampudia C, Jilli L, Brunoud G, Mutterer J, Vallet E, Sakvarelidze-Achard L, Davière JM, Navarro-Galiano A, Walia A, Lazary S, Legrand J, Weinstain R, Jones AM, Prat S, Achard P, Vernoux T

Résumé

Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.

Mots clés

Gibberellins, metabolism, Meristem, metabolism, Signal Transduction, Arabidopsis, metabolism, Arabidopsis Proteins, metabolism, Biosensing Techniques, Gene Expression Regulation, Plant, Plant Growth Regulators, metabolism, Plant Shoots, metabolism, Plants, Genetically Modified

Référence

Nat Commun. 2024 05 8;15(1):3895