Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells.

Date publication

juillet 2013

Auteurs

Membres identifiés du Cancéropôle Est :
Pr WEISS Etienne

Tous les auteurs :
Freund G, Sibler AP, Desplancq D, Oulad-Abdelghani M, Vigneron M, Gannon J, Van Regenmortel MH, Weiss E

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/23765067

Résumé

Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By following the fate of monoclonal antibodies that bind to nuclear antigens, it was possible to image endogenous targets and to show that inhibitory antibodies are able to induce cell growth suppression or cell death. Our electrotransfer system allowed the cancer cells we studied to be transduced without loss of viability and may have applications for a variety of intracellular immuno-interventions.

Référence

MAbs. 2013 Jul-Aug;5(4):518-22