Fiche publication
Date publication
novembre 2017
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOMBARDA Elisa
Tous les auteurs :
Kempf O, Kempf K, Schobert R, Bombarda E
Lien Pubmed
Résumé
Dark fluorescence quenchers are nonfluorescent dyes that can modulate the fluorescence signal of an appropriate fluorophore donor in a distance-dependent manner. Dark quenchers are extensively used in many biomolecular analytical applications, such as studies with fluorogenic protease substrates or nucleic acids probes. A very popular dark fluorescence quencher is dabcyl, which is a hydrophobic azobenzene derivative. However, its insolubility in water may constitute a major drawback, especially during the investigation of biochemical systems whose natural solvent is water. We designed and synthesized a new azobenzene-based dark quencher with excellent solubility in aqueous media, which represents a superior alternative to the much-used dabcyl. The advantage of hydrodabcyl over dabcyl is exemplarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the proteases thermolysin and papain.
Référence
Anal Chem. 2017 11 21;89(22):11893-11897
