Fiche publication
Date publication
novembre 2017
Journal
Photochemistry and photobiology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOMBARDA Elisa
Tous les auteurs :
Bombarda E, Ullmann GM
Lien Pubmed
Résumé
In this work, we calculate the protonation probabilities of titratable residues of bovine rhodopsin using the Poisson-Boltzmann equation. We also consider the influence of the membrane potential. Our results indicate that at physiological pH, the titratable groups directly involved in photosensing, namely Glu113, Glu181 and the retinal Schiff base, are charged. In contrast, the residues Asp83, Glu122 and His211, which are buried in the membrane, are uncharged. However, as these later residues are localized in the middle of the membrane, they are exposed to the membrane potential more strongly, which may have important functional implications. Despite of their large distance, Asp83 and Glu122 interact relatively strongly. As these two residues are in contact with opposite sides of the membrane, the membrane potential has different effects on them, which allows an enhancement of the membrane potential signal. An analysis of the different contributions to the protonation energy indicates that conformational changes that reduce the desolvation penalty of Asp83, Glu122 and His211 may lead to a complex protonation pattern change that allows an influence of the membrane potential on the function of rhodopsin. The high degree of evolutionary conservation of these three buried residues supports the idea of their functional importance. Our results are in-line with many experimental findings and lead to new ideas that can be experimentally tested.
Mots clés
Animals, Cattle, Computer Simulation, Hydrogen-Ion Concentration, Membrane Potentials, Protons, Rhodopsin, chemistry, Static Electricity
Référence
Photochem Photobiol. 2017 11;93(6):1388-1398
