Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins.

Date publication

mars 2015

Journal

Chemical communications (Cambridge, England)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr DZIUBA Dmytro

Tous les auteurs :
Dziuba D, Pohl R, Hocek M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/25704490

Résumé

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene-cyanoacetamide moiety, were tethered to 2'-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.

Mots clés

Aniline Compounds, chemistry, Benzylidene Compounds, chemistry, DNA, chemistry, DNA-Binding Proteins, chemistry, DNA-Directed DNA Polymerase, metabolism, Fluorescent Dyes, chemistry, Molecular Structure, Nitriles, chemistry, Protein Binding

Référence

Chem Commun (Camb). 2015 03 21;51(23):4880-2