Fiche publication
Date publication
juillet 2024
Journal
Science advances
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TORA Laszlo
,
Pr DIDIER Pascal
Tous les auteurs :
Roozbahani GM, Colosi PL, Oravecz A, Sorokina EM, Pfeifer W, Shokri S, Wei Y, Didier P, DeLuca M, Arya G, Tora L, Lakadamyali M, Poirier MG, Castro CE
Lien Pubmed
Résumé
DNA origami nanostructures (DOs) are promising tools for applications including drug delivery, biosensing, detecting biomolecules, and probing chromatin substructures. Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing, visualizing, and controlling biomolecular processes within live cells. We present an approach to deliver DOs into live-cell nuclei. We show that these DOs do not undergo detectable structural degradation in cell culture media or cell extracts for 24 hours. To deliver DOs into the nuclei of human U2OS cells, we conjugated 30-nanometer DO nanorods with an antibody raised against a nuclear factor, specifically the largest subunit of RNA polymerase II (Pol II). We find that DOs remain structurally intact in cells for 24 hours, including inside the nucleus. We demonstrate that electroporated anti-Pol II antibody-conjugated DOs are piggybacked into nuclei and exhibit subdiffusive motion inside the nucleus. Our results establish interfacing DOs with a nuclear factor as an effective method to deliver nanodevices into live-cell nuclei.
Mots clés
Cell Nucleus, metabolism, Humans, DNA, chemistry, Nanostructures, chemistry, RNA Polymerase II, metabolism, Cell Line, Tumor, Nanotubes, chemistry
Référence
Sci Adv. 2024 07 5;10(27):eadn9423
