Fiche publication
Date publication
août 2024
Journal
Developmental cell
Auteurs
Membres identifiés du Cancéropôle Est :
Pr RIVELINE Daniel
Tous les auteurs :
Mukenhirn M, Wang CH, Guyomar T, Bovyn MJ, Staddon MF, van der Veen RE, Maraspini R, Lu L, Martin-Lemaitre C, Sano M, Lehmann M, Hiraiwa T, Riveline D, Honigmann A
Lien Pubmed
Résumé
Formation of fluid-filled lumina by epithelial tissues is essential for organ development. How cells control the hydraulic and cortical forces to control lumen morphology is not well understood. Here, we quantified the mechanical role of tight junctions in lumen formation using MDCK-II cysts. We found that the paracellular ion barrier formed by claudin receptors is not required for the hydraulic inflation of a lumen. However, the depletion of the zonula occludens scaffold resulted in lumen collapse and folding of apical membranes. Combining quantitative measurements of hydrostatic lumen pressure and junctional tension with modeling enabled us to explain lumen morphologies from the pressure-tension force balance. Tight junctions promote lumen inflation by decreasing cortical tension via the inhibition of myosin. In addition, our results suggest that excess apical area contributes to lumen opening. Overall, we provide a mechanical understanding of how epithelial cells use tight junctions to modulate tissue and lumen shape.
Mots clés
biological physics, lumen morphogenesis, lumen pressure, mechano-biology, myosin contractility, tight junction, tissue hydraulics
Référence
Dev Cell. 2024 08 8;:
