Polyphenol-rich juice sustains eNOS activation through phosphorylation and expression via redox-sensitive pathways in endothelial cells.

Fiche publication

Date publication

septembre 2024

Journal

Food science and biotechnology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr SCHINI-KERTH Valérie , Dr AUGER Cyril

Tous les auteurs :
Kim JH, Choi MS, Auger C, Lee KW, Schini-Kerth VB

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/39184991

Résumé

A sustained formation of nitric oxide (NO) by endothelial nitric oxide synthase (eNOS) is crucial to safeguard the vascular system against the development of cardiovascular diseases. This study investigated the prolonged phosphorylation and expression of eNOS induced by polyphenol-rich juice (AMJ), along with its underlying mechanisms. The findings revealed that AMJ triggered concentration- and time-dependent increases in eNOS phosphorylation and expression, leading to sustained NO production for 15 h. Investigations with various enzymes and inhibitors revealed that the effect of AMJ was associated with redox sensitivity, activating the PI3-kinase/Akt, JNK, and p38 MAPK pathways. These pathways led to the inactivation of transcription factors FoxO1 and FoxO3a through phosphorylation, relieving their repression on eNOS expression. Therefore, the capability of AMJ to consistently trigger prolonged eNOS phosphorylation and expression via complex redox-sensitive pathways highlights its potential for maintaining vascular health and preventing cardiovascular diseases.