Fiche publication
Date publication
juillet 2015
Journal
Materials (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHAN-SENG Delphine
Tous les auteurs :
Neto MF, Letteri R, Chan-Seng D, Emrick T, Figueiredo ML
Lien Pubmed
Résumé
Successful gene delivery to skeletal muscle is a desirable goal, not only for treating muscle diseases, but also for immunization, treatment of metabolic disorders, and/or delivering gene expression that can treat systemic conditions, such as bone metastatic cancer, for example. Although naked DNA uptake into skeletal muscle is possible, it is largely inefficient in the absence of additional chemical or physical delivery methods. We describe a system for delivery of non-viral or plasmid DNA to skeletal muscle using ultrasound-assisted sonoporation of a nanoplex combining plasmid DNA and a branched polymer based on poly(cyclooctene-oligopeptide). The materials and methods described herein promise to advance the field of sonodelivery and of gene delivery to muscle for therapeutic applications since a simple system is presented that enables long-term gene expression with the promise of a minimal inflammatory gene expression profile.
Mots clés
episome, luciferase, microbubbles, nanoplex, non-viral vector, polymer, skeletal muscle, sonodelivery, sustained expression
Référence
Materials (Basel). 2015 07 1;8(7):4608-4617
