Fiche publication
Date publication
novembre 2014
Journal
Journal of cell science
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BAGUET Aurélie
,
Dr TOMASETTO Catherine
Tous les auteurs :
Cougot N, Daguenet E, Baguet A, Cavalier A, Thomas D, Bellaud P, Fautrel A, Godey F, Bertrand E, Tomasetto C, Gillet R
Lien Pubmed
Résumé
Metastatic lymph node 51 (MLN51, also known as CASC3) is a core component of the exon junction complex (EJC), which is loaded onto spliced mRNAs and plays an essential role in determining their fate. Unlike the three other EJC core components [eIF4AIII, Magoh and Y14 (also known as RBM8A)], MLN51 is mainly located in the cytoplasm, where it plays a key role in the assembly of stress granules. In this study, we further investigated the cytoplasmic role of MLN51. We show that MLN51 is a new component of processing bodies (P-bodies). When overexpressed, MLN51 localizes in novel small cytoplasmic foci. These contain RNA, show directed movements and are distinct from stress granules and P-bodies. The appearance of these foci correlates with the process of P-body disassembly. A similar reduction in P-body count is also observed in human HER2-positive (HER2(+)) breast cancer cells overexpressing MLN51. This suggests that P-body disassembly and subsequent mRNA deregulation might correlate with cancer progression.
Mots clés
Breast Neoplasms, genetics, Cytoplasm, metabolism, Cytoplasmic Granules, genetics, Eukaryotic Initiation Factor-4A, genetics, HeLa Cells, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Neoplasm Proteins, genetics, Nuclear Proteins, genetics, RNA, Messenger, metabolism, RNA-Binding Proteins, genetics
Référence
J. Cell. Sci.. 2014 Nov;127(Pt 21):4692-701
