Fiche publication
Date publication
septembre 2024
Journal
Bioconjugate chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah
,
Dr REMY Jean-Serge
,
Dr WAGNER Alain
,
Dr KICHLER Antoine
Tous les auteurs :
Ripoll M, Cahuzac H, Dovgan I, Ursuegui S, Neuberg P, Erb S, Cianférani S, Kichler A, Remy JS, Wagner A
Lien Pubmed
Résumé
RNA interference is a widely used biological process by which double-stranded RNA induces sequence-specific gene silencing by targeting mRNA for degradation. However, the physicochemical properties of siRNAs make their delivery extremely challenging, thus limiting their bioavailability at the target site. In this context, we developed a versatile and selective siRNA delivery system of a trastuzumab-conjugated nanocarrier. These immunoconjugates consist of the assembly by electrostatic interactions of an oligonucleotide-modified antibody with a cationic micelle for the targeted delivery of siRNA in HER2-overexpressing cancer cells. Results show that, when associated with the corresponding siRNA at the appropriate N/P ratio, our supramolecular assembly was able to efficiently induce luciferase and PLK-1 gene silencing in a cell-selective manner in vitro.
Référence
Bioconjug Chem. 2024 09 25;:
