Fiche publication
Date publication
septembre 2024
Journal
ACS synthetic biology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr WEISSMAN Kira
Tous les auteurs :
Massicard JM, Noel D, Calderari A, Le Jeune A, Pauthenier C, Weissman KJ
Lien Pubmed
Résumé
The filamentous are among the most prolific producers of bioactive natural products and are thus attractive chassis for the heterologous expression of native and designed biosynthetic pathways. Although suitable hosts exist, including genetically engineered cluster-free mutants, the approach is currently limited by the relative paucity of synthetic biology tools facilitating the de novo assembly of multicomponent gene clusters. Here, we report a modular system (MoClo) for including a set of adapted vectors and genetic elements, which allow for the construction of complete genetic circuits. Critical functional validation of each of the elements was obtained using the previously reported β-glucuronidase (GusA) reporter system. Furthermore, we provide proof-of-principle for the toolbox in, demonstrating the efficient assembly of a biosynthetic pathway to flavokermesic acid (FK), an advanced precursor of the commercially valuable carminic acid.
Mots clés
Streptomyces, anthraquinone, modular cloning, natural product, synthetic biology
Référence
ACS Synth Biol. 2024 09 22;:
