Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies.

Fiche publication

Date publication

septembre 2024

Journal

STAR protocols

Auteurs

Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain , Dr CHAUBET Guilhem

Tous les auteurs :
Rady T, Lehot V, Most J, Erb S, Cianferani S, Chaubet G, Basse N, Wagner A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/39342618

Résumé

Antibody-oligonucleotide conjugates (AOCs) are a fast-expanding modality for targeted delivery of therapeutic oligonucleotides to tissues. Here, we present a protocol to generate, purify, and analyze AOCs from off-the-shelf antibodies. We describe steps to conjugate single/double-stranded oligonucleotides bearing amine handles to linkers and, then, to antibodies using well-established chemistry. In addition, we provide details regarding the purification techniques and analytical methods suitable for AOC. This protocol can be applied for several purposes where AOC is a modality of interest. For complete details on the use and execution of this protocol, please refer to Rady et al..