Fiche publication
Date publication
octobre 2024
Journal
Small (Weinheim an der Bergstrasse, Germany)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SCHAAF Pierre
,
Dr SCHMUTZ Marc
Tous les auteurs :
More SH, Runser JY, Ontani A, Fores JR, Carvalho A, Blanck C, Serra CA, Schmutz M, Schaaf P, Jierry L
Lien Pubmed
Résumé
Enzymatically-active polyelectrolyte multilayers containing n layers of phosphatase (AP-PEM) induce the formation of supported biocatalytic supramolecular hydrogels when brought in contact with the precursor tripeptide Fmoc-FFpY (Fmoc = N-fluorenylmethyloxycarbonyl; F = Phenylalanine; Y = Tyrosine; p = phosphate group). AP-PEM triggers the spatially-localized hydrogelation reaching 2, 17 and 350 µm of thickness for n = 1, 2 and 3, respectively. As observed by cryo scanning electron microscopy, a dense nanofibrous network underpinning the hydrogel shows parallelly orientated Fmoc-FFY peptide-based fibrils, perpendicular to the substrate. For the gel generated by the AP-PEM, fluorescence confocal microscopy images show that during the peptide self-assembly, some enzymes are distributed in the hydrogel, preferentially located in few dozens of micrometers above the substrate. In addition, a self-assembly growth rate of 5 µm min is determined when the hydrogelation starts. Through transmission electron microscopy immuno-labelling experiments on self-assemblies generated in solution, we observe that AP are decorating the Fmoc-FFY nanofibers. It is observed both a long-term stability and a higher biocatalytic activity of the so AP-encapsulated hydrogel compared to the bare AP-PEM. This bioactivity can be tuned by the number n in batch and under continuous flow conditions. To illustrate the versatility of this enzyme-supported strategy, multi-catalytic transformations in continuous flow conditions have been successfully carried out using supported supramolecular hydrogel.
Mots clés
biocatalytic flow process, enzyme‐assisted self‐assembly, peptide‐based nanofiber, polyelectrolyte multilayer film, supramolecular hydrogel
Référence
Small. 2024 10 11;:e2405326
