Crystal structure of the GDP-bound GTPase Era from Staphylococcus aureus.

Fiche publication

Date publication

octobre 2024

Journal

Biochemical and biophysical research communications

Auteurs

Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat

Tous les auteurs :
Klochkova E, Biktimirov A, Islamov D, Belousov A, Validov S, Yusupov M, Usachev K

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/39432921

Résumé

GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.76 Å resolution crystal structure of Staphylococcus aureus Era in complex with GDP is presented. Structural comparison with other GTP-bound and GDP-bound homologous proteins, GTPase domain and the KH domain revealed a mutual orientation in S. aureus which has not been described before. The GDP-bound Era structure presented here will facilitate efforts to elucidate its interactions with its regulators and lay the foundation for a structure-based search for specific inhibitors.