Fiche publication
Date publication
octobre 2014
Journal
RNA (New York, N.Y.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MEIGNIN Carine
Tous les auteurs :
McDermott SM, Yang L, Halstead JM, Hamilton RS, Meignin C, Davis I
Lien Pubmed
Résumé
Localized mRNA translation is thought to play a key role in synaptic plasticity, but the identity of the transcripts and the molecular mechanism underlying their function are still poorly understood. Here, we show that Syncrip, a regulator of localized translation in the Drosophila oocyte and a component of mammalian neuronal mRNA granules, is also expressed in the Drosophila larval neuromuscular junction, where it regulates synaptic growth. We use RNA-immunoprecipitation followed by high-throughput sequencing and qRT-PCR to show that Syncrip associates with a number of mRNAs encoding proteins with key synaptic functions, including msp-300, syd-1, neurexin-1, futsch, highwire, discs large, and α-spectrin. The protein levels of MSP-300, Discs large, and a number of others are significantly affected in syncrip null mutants. Furthermore, syncrip mutants show a reduction in MSP-300 protein levels and defects in muscle nuclear distribution characteristic of msp-300 mutants. Our results highlight a number of potential new players in localized translation during synaptic plasticity in the neuromuscular junction. We propose that Syncrip acts as a modulator of synaptic plasticity by regulating the translation of these key mRNAs encoding synaptic scaffolding proteins and other important components involved in synaptic growth and function.
Mots clés
Animals, Blotting, Western, Cells, Cultured, Drosophila Proteins, genetics, Drosophila melanogaster, genetics, Immunoenzyme Techniques, Immunoprecipitation, Nerve Tissue Proteins, genetics, Neuromuscular Junction, cytology, RNA, Messenger, genetics, RNA-Binding Proteins, genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction
Référence
RNA. 2014 Oct;20(10):1593-606
