Fiche publication
Date publication
décembre 2024
Journal
STAR protocols
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MONCHAUD David
Tous les auteurs :
Mitteaux J, Monchaud D
Lien Pubmed
Résumé
The isolation of G-quadruplexes (G4s) from human cells using specific molecular tools constitutes an invaluable step forward in uncovering the biology of these higher-order DNA and RNA structures. Here, we present an improved version of the G4-RNA precipitation (G4RP) protocol developed to identify RNA G4s from human cancer cells. We describe steps for cell treatment and lysis, chemoprecipitation of G4s using TASQ tools, go/no-go steps, and quantitative reverse-transcription PCR (RT-qPCR) quantification and analysis. For complete details on the use and execution of this protocol, please refer to Mitteaux et al..
Mots clés
Biophysics, Chemistry, Genetics, Molecular Biology
Référence
STAR Protoc. 2024 12 10;5(4):103480
