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Date publication
janvier 2025
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BONNET Dominique
,
Dr DZIUBA Dmytro
Tous les auteurs :
Weiss L, Bonnet D, Dziuba D, Karpenko J
Lien Pubmed
Résumé
The worldwide spread of antibiotic resistance is considered to be one of the major health threats to society. While developing new antibiotics is crucial, there is also a strong need for next-generation analytical methods for studying the physiological state of live bacteria in heterogeneous populations and their response to environmental stress. Here we report a single-cell high-throughput method to monitor changes in the bacterial cell envelope in response to stress based on ratiometric flow cytometry. We used a sensitive fluorescent molecular probe, the Nile Red-based solvatochromic antimicrobial peptide with defined cellular localization in Gram-positive and Gram-negative bacteria. We also developed a robust protocol for calculating the generalized polarization (GP) of fluorescence adapted to flow cytometry. Our methodology enabled rapid detection of perturbations in the bacterial cell envelope caused by exposure to antibiotics, heat shock, and other factors.
Mots clés
Flow Cytometry, Fluorescent Dyes, chemistry, Gram-Negative Bacteria, cytology, Cell Wall, chemistry, Antimicrobial Peptides, chemistry, Gram-Positive Bacteria, Anti-Bacterial Agents, pharmacology, Cell Membrane, chemistry, Oxazines
Référence
Anal Chem. 2025 01 14;97(1):622-628
